Algorithms¶
Fragment Generation¶
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class
brainlit.algorithms.generate_fragments.state_generation(image_path: Union[str, Path], new_layers_dir: Union[str, Path], ilastik_program_path: str, ilastik_project_path: str, fg_channel: int = 0, chunk_size: List[float] = [375, 375, 125], soma_coords: List[list] = [], resolution: List[float] = [0.3, 0.3, 1], parallel: int = 1, prob_path: Union[str, Path] = None, fragment_path: Union[str, Path] = None, tiered_path: Union[str, Path] = None, states_path: Union[str, Path] = None)¶ This class encapsulates the processing that turns an image into a set of fragments with endpoints etc. needed to perform viterbrain tracing.
- Parameters:
image_path (str or pathlib.Path) -- Path to image zarr.
new_layers_dir (str or pathlib.Path) -- Path to directory where new layers will be written.
ilastik_program_path (str) -- Path to ilastik program.
ilastik_project_path (str) -- Path to ilastik project for segmentation of image.
fg_channel (int) -- Channel of image taken to be foreground.
chunk_size (List[float]) -- Chunk size too be used in parallel processing. Defaults to [375, 375, 125].
soma_coords (List[list]) -- List of coordinates of soma centers. Defaults to [].
resolution (List[float) -- Resolution of image in microns. Defaults to [0.3, 0.3, 1].
parallel (int) -- Number of threads to use for parallel processing. Defaults to 1.
prob_path (str or pathlib.Path) -- Path to alrerady computed probability image (ilastik output). Defaults to None.
fragment_path (str or pathlib.Path) -- Path to alrerady computed fragment image. Defaults to None.
tiered_path (str or pathlib.Path) -- Path to alrerady computed tiered image. Defaults to None.
states_path (str or pathlib.Path) -- Path to alrerady computed states file. Defaults to None.
- Raises:
ValueError -- Image must be four dimensional (cxyz)
ValueError -- Chunks must include all channels and be 4D.
ValueError -- Already computed images must match image in spatial dimensions.
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compute_bfs(self)¶ Compute bfs from highest degree node
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compute_edge_weights(self)¶ Create viterbrain object and compute edge weights
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compute_frags(self)¶ Compute all fragments for image
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compute_image_tiered(self)¶ Compute entire tiered image then reassemble and save as zarr
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compute_soma_lbls(self)¶ Compute fragment ids of soma coordinates.
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compute_states(self, alg: str = 'nb')¶ Compute entire collection of states
- Parameters:
alg (string, optional) -- algorithm to use for endpoint estimation. "nb" for neighborhood method, "pc" for principal curves method. Defaults to "nb"
- Raises:
ValueError -- erroneously computed endpoints of soma state
Connect Fragments¶
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class
brainlit.algorithms.connect_fragments.ViterBrain(G: nx.Graph, tiered_path: str, fragment_path: str, resolution: List[float], coef_curv: float, coef_dist: float, coef_int: float, parallel: int = 1)¶ -
compute_all_costs_dist(self, frag_frag_func: Callable, frag_soma_func: Callable)¶ Splits up transition computation tasks then assembles them into networkx graph
- Parameters:
frag_frag_func (function) -- function that computes transition cost between fragments
frag_soma_func (function) -- function that computes transition cost between fragments
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compute_all_costs_int(self)¶ Splits up transition computation tasks then assembles them into networkx graph
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frag_frag_dist(self, pt1: List[float], orientation1: List[float], pt2: List[float], orientation2: List[float], verbose: bool = False)¶ Compute cost of transition between two fragment states
- Parameters:
pt1 (list of floats) -- first coordinate
orientation1 (list of floats) -- orientation at first coordinate
pt2 (list of floats) -- second coordinate
orientation2 (list of floats) -- orientation at second coordinate
verbose (bool, optional) -- Print transition cost information. Defaults to False.
- Raises:
ValueError -- if an orientation is not unit length
ValueError -- if distance or curvature cost is nan
- Returns:
cost of transition
- Return type:
[float]
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frag_soma_dist(self, point: List[float], orientation: List[float], soma_lbl: int, verbose: bool = False)¶ Compute cost of transition from fragment state to soma state
- Parameters:
- Raises:
ValueError -- if either distance or curvature cost is nan
ValueError -- if the computed closest soma coordinate is not on the soma
- Returns:
cost of transition [list of floats]: closest soma coordinate
- Return type:
[float]
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shortest_path(self, coord1: List[int], coord2: List[int])¶ Compute coordinate path from one coordinate to another.
- Parameters:
- Raises:
ValueError -- if state sequence contains a soma state that is not at the end
- Returns:
list of voxel coordinates of path
- Return type:
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Trace Analysis¶
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brainlit.algorithms.trace_analysis.speed(x: np.ndarray, t: np.ndarray, c: np.ndarray, k: np.integer, aux_outputs: bool = False)¶ Compute the speed of a B-Spline.
The speed is the norm of the first derivative of the B-Spline.
- Parameters:
x -- A 1xL array of parameter values where to evaluate the curve. It contains the parameter values where the speed of the B-Spline will be evaluated. It is required to be non-empty, one-dimensional, and real-valued.
t -- A 1xm array representing the knots of the B-spline. It is required to be a non-empty, non-decreasing, and one-dimensional sequence of real-valued elements. For a B-Spline of degree k, at least 2k + 1 knots are required.
c -- A dxn array representing the coefficients/control points of the B-spline. Given n real-valued, d-dimensional points ::math::x_k = (x_k(1),...,x_k(d)), c is the non-empty matrix which columns are ::math::x_1^T,...,x_N^T. For a B-Spline of order k, n cannot be less than m-k-1.
k -- A non-negative integer representing the degree of the B-spline.
- Returns:
A 1xL array containing the speed of the B-Spline evaluated at x
- Return type:
speed
References: .. [Rbbccb9c002d5-1] Kouba, Parametric Equations.
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brainlit.algorithms.trace_analysis.curvature(x: np.ndarray, t: np.ndarray, c: np.ndarray, k: np.integer, aux_outputs: bool = False)¶ Compute the curvature of a B-Spline.
The curvature measures the failure of a curve, r(u), to be a straight line. It is defined as
\[k = \lVert \frac{dT}{ds} \rVert,\]where T is the unit tangent vector, and s is the arc length:
\[T = \frac{dr}{ds},\quad s = \int_0^t \lVert r'(u) \rVert du,\]where r(u) is the position vector as a function of time.
The curvature can also be computed as
\[k = \lVert r'(t) \times r''(t)\rVert / \lVert r'(t) \rVert^3.\]- Parameters:
x -- A 1xL array of parameter values where to evaluate the curve. It contains the parameter values where the curvature of the B-Spline will be evaluated. It is required to be non-empty, one-dimensional, and real-valued.
t -- A 1xm array representing the knots of the B-spline. It is required to be a non-empty, non-decreasing, and one-dimensional sequence of real-valued elements. For a B-Spline of degree k, at least 2k + 1 knots are required.
c -- A dxn array representing the coefficients/control points of the B-spline. Given n real-valued, d-dimensional points ::math::x_k = (x_k(1),...,x_k(d)), c is the non-empty matrix which columns are ::math::x_1^T,...,x_N^T. For a B-Spline of order k, n cannot be less than m-k-1.
k -- A non-negative integer representing the degree of the B-spline.
- Returns:
A 1xL array containing the curvature of the B-Spline evaluated at x
- Return type:
curvature
References: .. [Rce97e449f49f-1] Máté Attila, The Frenet–Serret formulas.
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brainlit.algorithms.trace_analysis.torsion(x: np.ndarray, t: np.ndarray, c: np.ndarray, k: np.integer, aux_outputs: bool = False)¶ Compute the torsion of a B-Spline.
The torsion measures the failure of a curve, r(u), to be planar. If the curvature k of a curve is not zero, then the torsion is defined as
\[\tau = -n \cdot b',\]where n is the principal normal vector, and b' the derivative w.r.t. the arc length s of the binormal vector.
The torsion can also be computed as
\[\tau = \lvert r'(t), r''(t), r'''(t) \rvert / \lVert r'(t) \times r''(t) \rVert^2,\]where r(u) is the position vector as a function of time.
- Parameters:
x -- A 1xL array of parameter values where to evaluate the curve. It contains the parameter values where the torsion of the B-Spline will be evaluated. It is required to be non-empty, one-dimensional, and real-valued.
t -- A 1xm array representing the knots of the B-spline. It is required to be a non-empty, non-decreasing, and one-dimensional sequence of real-valued elements. For a B-Spline of degree k, at least 2k + 1 knots are required.
c -- A dxn array representing the coefficients/control points of the B-spline. Given n real-valued, d-dimensional points ::math::x_k = (x_k(1),...,x_k(d)), c is the non-empty matrix which columns are ::math::x_1^T,...,x_N^T. For a B-Spline of order k, n cannot be less than m-k-1.
k -- A non-negative integer representing the degree of the B-spline.
- Returns:
A 1xL array containing the torsion of the B-Spline evaluated at x
- Return type:
torsion
References: .. [R8b689f5f8f91-1] Máté Attila, The Frenet–Serret formulas.
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class
brainlit.algorithms.trace_analysis.CubicHermiteChain(x: np.array, y: np.array, left_dydx: np.array, right_dydx: np.array, extrapolate=None)¶ A third order spline class (continuous piecewise cubic representation), that is fit to a set of positions and one-sided derivatives. This is not a standard spline class (e.g. b-splines), because the derivatives are not necessarily continuous at the knots.
A subclass of PPoly, a piecewise polynomial class from scipy.
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class
brainlit.algorithms.trace_analysis.GeometricGraph(df: pd.DataFrame = None, root=1)¶ The shape of the neurons are expressed and fitted with splines in this undirected graph class.
The geometry of the neurons are projected on undirected graphs, based on which the trees of neurons consisted for splines is constructed. It is required that each node has a loc attribute identifying that points location in space, and the location should be defined in 3-dimensional cartesian coordinates. It extends nx.Graph and rejects duplicate node input.
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fit_spline_tree_invariant(self, spline_type: Union[BSpline, CubicHermiteSpline] = BSpline, k=3)¶ Construct a spline tree based on the path lengths.
- Parameters:
spline_type -- BSpline or CubicHermiteSpline, spline type that will be fit to the data. BSplines are typically used to fit position data only, and CubicHermiteSplines can only be used if derivative, and independent variable information is also known.
- Raises:
ValueError -- check if every node is unigue in location
ValueError -- check if every node is assigned to at least one edge
ValueError -- check if the graph contains undirected cycle(s)
ValueErorr -- check if the graph has disconnected segment(s)
- Returns:
nx.DiGraph a parent tree with the longest path in the directed graph
- Return type:
spline_tree
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Soma Detection¶
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brainlit.algorithms.detect_somas.find_somas(volume: np.ndarray, res: list)¶ Find bright neuron somas in an input volume.
This simple soma detector assumes that somas are brighter than the rest of the objects contained in the input volume.
To detect somas, these steps are performed:
Check input volume shape. This detector requires the x and y dimensions of the input volumes to be larger than 20 pixels.
Zoom volume. We found that this simple soma detector works best when then input volume has size 160 x 160 x 50. We use ndimage.zoom to scale the input volume size to the desired shape.
Binarize volume. We use Otsu thresholding to binarize the image.
Erode the binarized image. We erode the binarized image with a structuring element which size is directly proportional to the maximum zoom factor applied to the input volume.
Remove unreasonable connected components. After erosion, we compute the equivalent diameter d of each connected component, and only keep those ones such that 5mu m leq d < 21 mu m
Find relative centroids. Finally, we compute the centroids of the remaining connected components. The centroids are in voxel units, relative to the input volume.
- Parameters:
volume (numpy.ndarray) -- The 3D image array to run the detector on.
res (list) -- A 1 x 3 list containing the resolution of each voxel in nm.
- Returns:
label (bool) -- A boolean value indicating whether the detector found any somas in the input volume.
rel_centroids (numpy.ndarray) -- A N x 3 array containing the relative voxel positions of the detected somas.
out (numpy.ndarray) -- A 160 x 160 x 50 array containing the detection mask.
Examples
>>> # download a volume >>> dir = "s3://open-neurodata/brainlit/brain1" >>> dir_segments = "s3://open-neurodata/brainlit/brain1_segments" >>> volume_keys = "4807349.0_3827990.0_2922565.75_4907349.0_3927990.0_3022565.75" >>> mip = 1 >>> ngl_sess = NeuroglancerSession( >>> mip=mip, url=dir, url_segments=dir_segments, use_https=False >>> ) >>> res = ngl_sess.cv_segments.scales[ngl_sess.mip]["resolution"] >>> volume_coords = np.array(os.path.basename(volume_keys).split("_")).astype(float) >>> volume_vox_min = np.round(np.divide(volume_coords[:3], res)).astype(int) >>> volume_vox_max = np.round(np.divide(volume_coords[3:], res)).astype(int) >>> bbox = Bbox(volume_vox_min, volume_vox_max) >>> img = ngl_sess.pull_bounds_img(bbox) >>> # apply soma detector >>> label, rel_centroids, out = find_somas(img, res)